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What is the cell line Lena used for her first formal differentiation attempt (per lab notebook LH-EXP-23-003), and what was the date of that attempt? For context, name also the different cell line she had thawed for general hiPSC maintenance six days earlier (per LH-EXP-23-001).

Gold answer

First differentiation attempt: cell line hESC-NS-12 on 2023-09-11 (lab notebook LH-EXP-23-003). The earlier general-maintenance thaw on 2023-09-05 (LH-EXP-23-001) used a different line, R-H-42b.

Filesystem View raw JSON
Input
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Output
2k
Steps
1
Tools
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Latency
33.3s
Cost
$2.84
#1 Ran rg --files agent_memory 13,068 chars
Command command_execution 
rg --files agent_memory
Result 7 lines 
agent_memory/communications/f173_confusing_results_email.md
agent_memory/communications/f156_mcb_orientation_schedule.md
agent_memory/communications/f178_microscopy_question.md
agent_memory/communications/f193_it_network_maintenance.md
agent_memory/communications/f181_lab_lunch_planning.md
agent_memory/communications/f180_bio510_project_submission.md
agent_memory/communications/f188_conference_abs…
#1 Ran rg -n "LH-EXP-23-00[13]|formal differentiation|first formal differentiation|thawed|hiPSC|maintenance|cell line|Lena" agent_memory 728,602 chars
Command command_execution 
rg -n "LH-EXP-23-00[13]|formal differentiation|first formal differentiation|thawed|hiPSC|maintenance|cell line|Lena" agent_memory
Result 6 lines 
agent_memory/communications/f174_troubleshooting_meeting_sched.md:20:Lena Hansen
agent_memory/communications/f174_troubleshooting_meeting_sched.md:36:Lena,
agent_memory/communications/f174_troubleshooting_meeting_sched.md:75:Lena Hansen
agent_memory/communications/f174_troubleshooting_meeting_sched.md:91:Lena,
agent_memory/communications/f174_troubleshooting_meeting_sched.md:124:Lena Hansen
agent_…
#1 Ran sed -n '1,180p' agent_memory/lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md 17,513 chars
Command command_execution 
sed -n '1,180p' agent_memory/lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md
Result 10 lines 
# Lab Notebook: LH-EXP-23-003 - Neuronal Differentiation - Attempt 1

**Date:** 2023-09-11
**Author:** Lena Hansen
**Project:** Core Project - hiPSC Neuronal Differentiation
**Experiment Series:** experiment_series_lh_23

## Overview

This marks the beginning of the first formal attempt at inducing neuronal differentiation from the established human induced pluripotent stem cell (hiPSC) line, hESC…
#1 Ran sed -n '1,180p' agent_memory/lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md 27,617 chars
Command command_execution 
sed -n '1,180p' agent_memory/lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md
Result 10 lines 
# Lab Notebook Entry: LH-EXP-23-001 - First Thaw

**Date:** 2023-09-05
**Author:** Lena Hansen
**Project:** Rotation Project - hiPSC Maintenance and Differentiation
**Location:** Maple Creek Lab, Cell Culture Suite 2B

## Objective

This entry details the first successful thaw and plating of human induced pluripotent stem cells (hiPSCs), specifically line R-H-42b. The primary goal was to re-establ…
#1 Ran nl -ba agent_memory/lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md | sed -n '1,35p' 2,607 chars
Command command_execution 
nl -ba agent_memory/lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md | sed -n '1,35p'
Result 10 lines 
     1	# Lab Notebook: LH-EXP-23-003 - Neuronal Differentiation - Attempt 1
     2	
     3	**Date:** 2023-09-11
     4	**Author:** Lena Hansen
     5	**Project:** Core Project - hiPSC Neuronal Differentiation
     6	**Experiment Series:** experiment_series_lh_23
     7	
     8	## Overview
     9	
    10	This marks the beginning of the first formal attempt at inducing neuronal differentiation from …
#1 Ran nl -ba agent_memory/lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md | sed -n '1,40p' 4,241 chars
Command command_execution 
nl -ba agent_memory/lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md | sed -n '1,40p'
Result 10 lines 
     1	# Lab Notebook Entry: LH-EXP-23-001 - First Thaw
     2	
     3	**Date:** 2023-09-05
     4	**Author:** Lena Hansen
     5	**Project:** Rotation Project - hiPSC Maintenance and Differentiation
     6	**Location:** Maple Creek Lab, Cell Culture Suite 2B
     7	
     8	## Objective
     9	
    10	This entry details the first successful thaw and plating of human induced pluripotent stem cells …
Answer
Lena’s first formal differentiation attempt used hiPSC line `hESC-NS-12`, and the attempt was dated `2023-09-11` in [LH-EXP-23-003](/Users/prasanna/Documents/supermemoryai/supermemoryfs/.scratch/eval/eval/workspace/fs/dp_007/agent_memory/lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md:3). Six days earlier, in [LH-EXP-23-001](/Users/prasanna/Documents/supermemoryai/supermemoryfs/.scratch/eval/eval/workspace/fs/dp_007/agent_memory/lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md:…
Judge: covers all asked facts
SMFS Won View raw JSON
Input
214k
Output
3k
Steps
1
Tools
8
Latency
1.4m
Cost
$2.65
#1 Ran /bin/zsh -lc pwd 108 chars
Command command_execution 
/bin/zsh -lc pwd
Result 2 lines 
/Users/prasanna/Documents/supermemoryai/supermemoryfs/.scratch/eval/eval/workspace/smfs/dp_007/agent_memory
#1 Ran test -f profile.md && sed -n '1,120p' profile.md || true 158 chars
Command command_execution 
test -f profile.md && sed -n '1,120p' profile.md || true
Result 6 lines 
# Memory Profile
# This file is auto-generated from your memories.
# It is not editable. To update, modify the source files
# that contain this information.
#1 Ran rg --files 10,479 chars
Command command_execution 
rg --files
Result 10 lines 
profile.md
meetings/mentor_1on1/20231129_tanaka_notes.md
meetings/mentor_1on1/20231115_tanaka_notes.md
meetings/mentor_1on1/20231101_tanaka_notes.md
meetings/mentor_1on1/20231018_tanaka_notes.md
meetings/mentor_1on1/20231004_tanaka_notes.md
meetings/mentor_1on1/20230920_tanaka_notes.md
meetings/mentor_1on1/20230906_tanaka_notes.md
meetings/advisor_1on1/20231201_reed_notes.md
meetings/advisor_1on1/…
#1 Ran sed -n '1,220p' lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md 20,736 chars
Command command_execution 
sed -n '1,220p' lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md
Result 10 lines 
# Lab Notebook: LH-EXP-23-003 - Neuronal Differentiation - Attempt 1

**Date:** 2023-09-11
**Author:** Lena Hansen
**Project:** Core Project - hiPSC Neuronal Differentiation
**Experiment Series:** experiment_series_lh_23

## Overview

This marks the beginning of the first formal attempt at inducing neuronal differentiation from the established human induced pluripotent stem cell (hiPSC) line, hESC…
#1 Ran sed -n '1,220p' lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md 28,609 chars
Command command_execution 
sed -n '1,220p' lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md
Result 10 lines 
# Lab Notebook Entry: LH-EXP-23-001 - First Thaw

**Date:** 2023-09-05
**Author:** Lena Hansen
**Project:** Rotation Project - hiPSC Maintenance and Differentiation
**Location:** Maple Creek Lab, Cell Culture Suite 2B

## Objective

This entry details the first successful thaw and plating of human induced pluripotent stem cells (hiPSCs), specifically line R-H-42b. The primary goal was to re-establ…
#1 Ran rg -n "cell line|Cell line|line|thaw|differentiation|formal|attempt|Date|date" lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.… 20,685 chars
Command command_execution 
rg -n "cell line|Cell line|line|thaw|differentiation|formal|attempt|Date|date" lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md
Result 2 lines 
lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md:3:**Date:** 2023-09-11
lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md:10:This marks the beginning of the first formal attempt at inducing neuronal differentiation from the established human induced pluripotent stem cell (hiPSC) line, hESC-NS-12, using the **Reed Lab hiPSC Neuronal Differentiation Protocol v3.2** (inte…
#1 Ran nl -ba lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md | sed -n '1,35p;130,140p' 3,284 chars
Command command_execution 
nl -ba lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md | sed -n '1,35p;130,140p'
Result 10 lines 
     1	# Lab Notebook: LH-EXP-23-003 - Neuronal Differentiation - Attempt 1
     2	
     3	**Date:** 2023-09-11
     4	**Author:** Lena Hansen
     5	**Project:** Core Project - hiPSC Neuronal Differentiation
     6	**Experiment Series:** experiment_series_lh_23
     7	
     8	## Overview
     9	
    10	This marks the beginning of the first formal attempt at inducing neuronal differentiation from …
#1 Ran nl -ba lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md | sed -n '1,45p;54,62p' 6,700 chars
Command command_execution 
nl -ba lab/notebook/20230905_LH-EXP-23-001_First-Thaw.md | sed -n '1,45p;54,62p'
Result 10 lines 
     1	# Lab Notebook Entry: LH-EXP-23-001 - First Thaw
     2	
     3	**Date:** 2023-09-05
     4	**Author:** Lena Hansen
     5	**Project:** Rotation Project - hiPSC Maintenance and Differentiation
     6	**Location:** Maple Creek Lab, Cell Culture Suite 2B
     7	
     8	## Objective
     9	
    10	This entry details the first successful thaw and plating of human induced pluripotent stem cells …
Answer
Lena’s first formal differentiation attempt used **hESC-NS-12** and was dated **2023-09-11** in [LH-EXP-23-003](/Users/prasanna/Documents/supermemoryai/supermemoryfs/.scratch/eval/eval/workspace/smfs/dp_007/agent_memory/lab/notebook/20230911_LH-EXP-23-003_Differentiation-Attempt1.md:3). Six days earlier, on **2023-09-05**, she had thawed **R-H-42b** for general hiPSC maintenance in [LH-EXP-23-001](/Users/prasanna/Documents/supermemoryai/supermemoryfs/.scratch/eval/eval/workspace/smfs/dp_007/age…
Judge: covers all asked facts